Thermo Scientific Pierce ECL Plus Substrate is an acridanbased chemiluminescent and chemifluorescent HRP substrate for Western blot detection using Xray film or CCD or laserbased imagers. This product is sold as Pierce ECL 2 Substrate ECL Plus Western blotting detection is optimized for use with HybondTMP PVDF membrane where the performance over ECL Western blotting is most enhanced. the duration of signal from ECL Plus A definitive advantage of the ECL Plus reagents over standard ECL reagents is the extended signal duration.
For example, developing a blot with ECL over time intervals like 1, 5, 10, 20, and 60 min (total time 96 minutes) gives an increasing signal intensity, but then the signal decreases. ECL Plus Western Blotting Detection Reagents immobilized specific antigens conjugated to Horseradish Peroxidase labelled antibodies. Product Booklet Codes: RPN2132 RPN2133. 2 Page finder 1. Legal 3 2. Handling 4 2. 1. Safety warnings and precautions 4 ECL Plus can be used with both nitrocellulose and PVDF Product specification ECL Plus Western blotting reagent pack RPN 2124 Before using this product, please read the instructions below for safe handling, storage and disposal.
Amersham ECL Rpn2132 ecl plus manual Western Blotting detection reagent from GE provides high sensitivity for chemiluminescent detection of immobilized specific antigens conjugated to Horseradish Peroxidase (HRP) labeled antibodies. 2. 1 Introduction Chemiluminescence is defined as light emission produced in a Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.
Due to the improved sensitivity of ECL Plus compared to ECL, optimization details as set out in the RPN booklets and TechTips No. 169 available from GE Healthcare are recommended. Step Action Notes A full range of products is available for Western blotting including ECL, ECL Plus, ECL Advance and ECL Plex Western blotting systems.
Fig 7. Transfer of E. Coli extracts using the TE 77 PWR instrument followed by detection of E. Coli GroEL on Hybond P membrane using the ECL View and Download Eclipse Fit clip user manual online. Fit clip MP3 Player pdf manual download.