Part 5: Gram Stain Procedure. Place slide with heat fixed smear on staining tray. In an actual laboratory setting, there are certain important steps that are not necessarily applicable in a virtual lab: Always wear gloves, and lab coat. Tie your hair properly to prevent any contamination from the culture you are working with. True to its name, the simple stain is a very simple staining procedure involving single solution of stain. Any basic dye such as methylene blue, safranin, or crystal violet can be used to color the bacterial cells.
Differential staining such as Gram Staining and AFB Staining are commonly used to identify and differentiate the bacterial Briefly describe the mechanism of Gram staining.
The Materials and Methods section should include a description of the proceduresteps in the Gram stain, including the preparation of a bacterial smear and heat fixation (3 points). Name the steps of the gram stain procedure, along with the timing of each Expert Answer Gram staining procedure is mainly used to differentiate gram positive bacteria and gram negative bacteria. THE GRAM STAIN The gram stain, originally developed in 1884 by Christian Gram, is probably the most important procedure in all of microbiology.
It has to be one of the most repeated procedures Jan 22, 2010 How to Gram Stain. Gram staining is a quick procedure used to look for the presence of bacteria in tissue samples and to characterise bacteria as Grampositive or Gramnegative, based on the chemical and physical properties of their cell Simple Stain Procedure: Procedure: 1. Clean and dry microscope slides thoroughly. 2.
Flame the surface in which the smear is to be spread. 3. Flame the inoculating loop. 4. Transfer a loop full of tap water to the flamed slide surface. 5. Reflame the loop making sure the entire length of the wire that will enter the tube has been heated to redness The Gram staining procedure involves four basic steps: 1. Shake off the excess water but do not blot dry between steps.
c. Stain with Gram's iodine solution for one minute and gently wash B. THE CAPSULE STAIN. DISCUSSION Many bacteria secrete a slimy, Differential staining is a procedure that takes advantage of differences in the physical and chemical properties of different groups of bacteria. It allows us to Cultures for Gram staining should be grown in media low in sugars to avoid the formation of acidic end products during cell growth.
Steps of the gram-staining procedure manual Staining: A. Add crystal violet stain over the fixed culture. Let stand for 10 to 60 seconds; for thinly prepared slides, it is usually acceptable to pour the stain on and off immediately.
This staining procedure differentiates microbes into two basic groups: Gram positive microbes and Gram negative Repeat steps 47 for each slant culture. 8. For broth cultures (yeast suspension), after flame sterilizing your loop, simply place a loopful above and save for Gram staining.
Simple Stain. 1. Cover the E. coli and S. aureus Gram stain or Gram staining, There are four basic steps of the Gram stain: Applying a primary stain (crystal violet) to a heatfixed smear of a bacterial culture.
Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they don't rinse out during the staining procedure;